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KMID : 0368420020450030177
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Journal of Plant Biology
2002 Volume.45 No. 3 p.177 ~ p.181
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Callus Induction and Plant Regeneration from Broccoli(Brassica oleracea var. italica) for Transformation
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Kim Jong-Hee
J. R. Botella
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Abstract
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We evaluated the efficiency of callus induction and plantlet regeneration from hypocotyl explants of broccoli (Brassica oleracea var. italica). The cultivars were ´Marathon´, ´Greenbelt´, and ´Shogun´. Transformation success was not affected by the presence of tobacco feeder-cell layers on the culture media. The frequency of shoot regeneration was greater from 10-d-old hypocotyls than from 14-d-old hypocotyls. Both ´Marathon´ and ´Greenbelt´ had higher potentials for tissue regeneration than did ´Shogun´. We found that for transformation selection, the optimum concentration was either 50 ㎎/L kanamycin or 100 ㎎/L geneticin.
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